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High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
 

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Article DOI: 10.3791/60378-v • 14:09 min • November 16th, 2019
November 16th, 2019 •
1, 1, 2, 1, 1
1Department of Physics and Technology, UiT The Arctic University of Norway, 2Vascular Biology Research Group, Department of Medical Biology, UiT The Arctic University of Norway

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Chip-based super-resolution optical microscopy is a novel approach to fluorescence microscopy and offers advantages in cost effectiveness and throughput. Here, the protocols for chip preparation and imaging are shown for TIRF microscopy and localization-based super-resolution microscopy.

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High-Throughput Total Internal Reflection Fluorescence Direct Stochastic Optical Reconstruction Microscopy Photonic Chip TIRF Microscopy Single-molecule Localization Microscopy Evanescent Waves Penetration Depth TIRF Illumination Optical Waveguide High-throughput Imaging Photonic Chip-based Illumination Imaging Objective Lens Decoupling Illumination And Collection Light Glass Petri Dish Wafer Tweezer Hellmanex Solution Hotplate
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