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Developmental Biology

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JoVE Journal Developmental Biology
Clonal Genetic Tracing using the Confetti Mouse to Study Mineralized Tissues
 

Clonal Genetic Tracing using the Confetti Mouse to Study Mineralized Tissues

Article DOI: 10.3791/60424-v • 07:41 min • October 23rd, 2019
October 23rd, 2019 •
1, 1, 1,2, 1,3
1Department of Physiology and Pharmacology, Karolinska Institutet, 2Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 3Department of Women's and Children's Health, Karolinska Institutet and Pediatric Endocrinology Unit, Karolinska University Hospital

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résumé
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This method describes the use of the R26R-Confetti (Confetti) mouse model to study mineralized tissues, covering all steps from the breeding strategy to the image acquirements. Included is a general protocol that can be applied to all soft tissues and a modified protocol that can be applied to mineralized tissues.

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Clonal Genetic Tracing Confetti Mouse Mineralized Tissues Fluorescent Proteins Biological Processes Regenerative Medicine Developmental Biology Cre Line Step-by-step Instructions Fluorescent Signals Mouse Tissue Imaging Soft Tissues Mineralized Tibiae And Femora Formaldehyde PBS 10% EDTA Solution Sodium Hydroxide
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