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Cancer Research

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JoVE Journal Cancer Research
Ultrasound-Guided Orthotopic Implantation of Murine Pancreatic Ductal Adenocarcinoma
 

Ultrasound-Guided Orthotopic Implantation of Murine Pancreatic Ductal Adenocarcinoma

Article DOI: 10.3791/60497-v • 06:21 min • November 19th, 2019
November 19th, 2019 •
1, 2,3, 2,3, 2,3, 1,4
1Department of Medicine, Perelman School of Medicine, University of Pennsylvania, 2Pancreatic Cancer Mouse Hospital, Perelman School of Medicine, University of Pennsylvania, 3Abramson Cancer Center, Perelman School of Medicine, University of Pennsylvania, 4Parker Institute for Cancer Immunotherapy, Perelman School of Medicine, University of Pennsylvania

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We describe a protocol for the ultrasound guided implantation of murine-derived pancreatic ductal adenocarcinoma cell lines directly into the native tumor site. This approach resulted in pancreatic tumors detectable by ultrasound scanning within 2-4 weeks of injection, and significantly reduced the proportion tumor cell seeding on the peritoneal wall as compared to surgical orthotopic implantation.

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Ultrasound-guided Orthotopic Implantation Murine Pancreatic Ductal Adenocarcinoma Study Native Tissue Site Inflammation Tumor Cell Injection High Throughput Method Large Cohorts Of Mice Ultrasound Guidance Abdominal Surgery Peritoneal Wall Seeding Histological Features Immunobiological Features Pancreatic Tumor Microenvironment Novel Therapeutic Combinations Clinically Relevant Setting Immuno-oncology Cancer Biology Therapies Normal Pancreatic Function Diabetes Real-time Ultrasound Imaging Needle Insertion Injection Technique
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