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Kwantificering van proliferative en dode cellen in Enteroïden
 
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Kwantificering van proliferative en dode cellen in Enteroïden

Article DOI: 10.3791/60501-v • 10:47 min • January 31st, 2020
January 31st, 2020 •
*1, *1, *1, 1, 1, 1, 1, 2, 2,3, 1, 1, 1
1Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genomic Resource Center, Medical College of Soochow University, Department of Oncology, The First Affiliated Hospital of Soochow University, 2Department of Pathology, University of Chicago, 3Department of Pathology, Brigham and Women's Hospital–Harvard Medical School
* These authors contributed equally

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Het gepresenteerde protocol maakt gebruik van flow cytometrie om het aantal vermenigvuldigende en dode cellen in gekweekte muisenteroïden te kwantificeren. Deze methode is nuttig om de effecten van medicamenteuze behandeling op organoïde proliferatie en overleving te evalueren.

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Biologie Kwestie 155 enteroïden darm stroomcytometrie proliferatie EdU propidiumjodide
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