Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Developmental Biology

Un abonnement à JoVE est nécessaire pour afficher ce contenu. Connectez-vous ou commencez votre essai gratuit.

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
 

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Article DOI: 10.3791/60647-v 08:30 min January 7th, 2020
January 7th, 2020

Chapitres

résumé

Here we presented a multiplexed single cell mRNA sequencing method to profile gene expression in mouse embryonic tissues. The droplet-based single cell mRNA sequencing (scRNA-Seq) method in combination with multiplexing strategies can profile single cells from multiple samples simultaneously, which significantly reduces reagent costs and minimizes experimental batch effects.

Tags

Keywords: Single Cell MRNA Sequencing Multiplexing Cell Isolation Cell Preparation Cell Counting Anchor Barcode Co-anchor Barcode Cell Filtering Chip Assembly Cell Solution Preparation Gel Bead Addition Partitioning Oil Chromium Controller Single Cell Analysis
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter