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Immunology and Infection

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JoVE Journal Immunology and Infection
Assessment of Acute Wound Healing using the Dorsal Subcutaneous Polyvinyl Alcohol Sponge Implantation and Excisional Tail Skin Wound Models.
 

Assessment of Acute Wound Healing using the Dorsal Subcutaneous Polyvinyl Alcohol Sponge Implantation and Excisional Tail Skin Wound Models.

Article DOI: 10.3791/60653-v • 09:06 min • March 25th, 2020
March 25th, 2020 •
1, 1, 1, 2, 1
1Department of Molecular Microbiology & Immunology, Brown University, 2Division of Surgical Research, Department of Surgery, Rhode Island Hospital and The Warren Alpert School of Medicine of Brown University

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Here, two murine wound healing models are described, one designed to assess cellular and cytokine wound healing responses and the other to quantify the rate of wound closure. These methods can be used with complex disease models such as diabetes to determine mechanisms of various aspects of poor wound healing.

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Acute Wound Healing Dorsal Subcutaneous Polyvinyl Alcohol Sponge Implantation Excisional Tail Skin Wound Models Wound Healing Response Cellular Kinetics Cytokine Kinetics Wound Closure PVA Sponge Model Tail Skin Excision Model Wound Leukocytes Phenotypic Analysis Functional Analysis Comorbid Conditions Diabetes Pneumonia Meredith Crane Bill Henry Research Assistant PVA Sponge Cutting Rehydration Autoclave Sterilization Sterile PBS Sterile Culture Dish Laminar Flow Hood Toe Pinch Response Anesthetized Mouse Povidone-iodine Solution
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