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Immunology and Infection

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JoVE Journal Immunology and Infection
A Novel High-Throughput Ex Vivo Ovine Skin Wound Model for Testing Emerging Antibiotics
 

A Novel High-Throughput Ex Vivo Ovine Skin Wound Model for Testing Emerging Antibiotics

Article DOI: 10.3791/64041-v • 08:30 min • September 16th, 2022
September 16th, 2022 •
1,2, 1,2, 1,2
1Sheffield Collaboratorium for Antimicrobial Resistance and Biofilms (SCARAB), University of Sheffield, 2Department of Chemical and Biological Engineering, University of Sheffield

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The protocol describes a step-by-step method to set up an ex vivo ovine wounded skin model infected with Staphylococcus aureus. This high-throughput model better simulates infections in vivo compared with conventional microbiology techniques and presents researchers with a physiologically relevant platform to test the efficacy of emerging antimicrobials.

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Keywords: Ovine Skin Wound Model High-throughput Ex Vivo Antimicrobial Efficacy Topical Antimicrobials Wound Infections Contamination Aseptic Technique Tissue Processing Skin Sample Chlorine Dioxide Solution Sterile PBS Air-liquid Interface
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