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Immunology and Infection

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JoVE Journal Immunology and Infection
Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses
 

Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses

Article DOI: 10.3791/60663-v • 08:10 min • January 15th, 2020
January 15th, 2020 •
1, 2, 1, 1, 1, 1, 1
1Central Laboratory, Hang Zhou Red Cross Hospital, 2Clinical Laboratory, Yuhang District Maternity and Child Health Care Hospital

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This protocol describes an experimental process to produce high-titer infectious viral pseudotyped particles (pp) with envelope glycoproteins from two influenza A strains and how to determine their infectivity. This protocol is highly adaptable to develop pps of any other type of enveloped viruses with different envelope glycoproteins.

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Production High-titer Infectious Influenza Pseudotyped Particles Envelope Glycoproteins Highly Pathogenic H5N1 Avian H7N9 Technique Research BSL-2 City PPS QRT-PCR Data Glycoprotein Expression Plasmid Virus Reassortment Cell Seeding Inverted Light Microscope Confluent Transfection Reagent Serum-free DMEM Plasmid DNA Reduced Serum Medium DNA Lipid Complex Incubation Period
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