JoVE Journal
Developmental Biology
Developmental Biology
Ce contenu est en libre accès.
Chapitres
résumé
Here, we describe the immunoprecipitation of ribosomes and associated RNA from select populations of adult male mouse germ cells using the RiboTag system. Strategic breeding and careful immunoprecipitation result in clean, reproducible results that inform on the germ cell translatome and provide insight into the mechanisms of mutant phenotypes.