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Developmental Biology

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JoVE Journal Developmental Biology
Endokardiyal ve Koroner Endotel Hücrelerinin Sıçan Kalbinin Ventriküler Serbest Duvarından İzolasyon
 
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Endokardiyal ve Koroner Endotel Hücrelerinin Sıçan Kalbinin Ventriküler Serbest Duvarından İzolasyon

Article DOI: 10.3791/61126-v • 08:22 min • April 15th, 2020
April 15th, 2020 •
1,2,3, 1,2,3, 1,2,3,4,5, 1,2,3,4,5
1Department of Pediatrics, Division of Cardiology, Stanford School of Medicine, 2Vera Moulton Wall Center for Pulmonary Vascular Disease, Stanford School of Medicine, 3Stanford Cardiovascular Institute, Stanford School of Medicine, 4Division of Pulmonary Biology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, 5Center for Stem Cell and Organoid Medicine, CuSTOM, Division of Developmental Biology, and Perinatal Institute, Cincinnati Children's Hospital Medical Center

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Biz bir sindirim tampon ardışık doku sindirim yoluyla sıçan kalplerinden endokardiyal ve koroner endotel hücrelerinin izolasyon için bir protokol sunmak, tekrarlayan santrifüj döngüleri hücre toplama, ve anti-sıçan CD31 mikroboncuklar kullanarak hücre arıtma.

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Gelişim biyolojisi Sayı 158 endokardiyum koroner endotel izolasyon kalp sıçan CD31
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