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Bioengineering

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Fabrication of Zero Mode Waveguides for High Concentration Single Molecule Microscopy
 

Fabrication of Zero Mode Waveguides for High Concentration Single Molecule Microscopy

Article DOI: 10.3791/61154-v 08:01 min May 12th, 2020
May 12th, 2020

Chapitres

résumé

Described here is a nanosphere lithography method for parallel fabrication of zero mode waveguides, which are arrays of nanoapertures in a metal-clad glass microscopy coverslip for single molecule imaging at nano- to micromolar concentrations of fluorophores. The method takes advantage of colloidal crystal self-assembly to create a waveguide template.

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Keywords: Zero Mode Waveguides Single Molecule Microscopy Nanosphere Lithography Evaporative Deposition Polystyrene Beads Humidity Chamber Optical Borosilicate Glass Coverslips Acetone Potassium Hydroxide Ethanol Centrifugation Relative Humidity
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