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JoVE Journal Bioengineering
Characterizing Single-Molecule Conformational Changes Under Shear Flow with Fluorescence Microscopy
 

Characterizing Single-Molecule Conformational Changes Under Shear Flow with Fluorescence Microscopy

Article DOI: 10.3791/60784-v • 08:47 min • January 25th, 2020
January 25th, 2020 •
1, *2, *3, 3, 1,2, 1,4
1Department of Bioengineering, Lehigh University, 2Department of Materials Science and Engineering, Lehigh University, 3Department of Chemistry, Lehigh University, 4Department of Mechanical Engineering and Mechanics, Lehigh University
* These authors contributed equally

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résumé
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We present a protocol for immobilizing single macromolecules in microfluidic devices and quantifying changes in their conformations under shear flow. This protocol is useful for characterizing the biomechanical and functional properties of biomolecules such as proteins and DNA in a flow environment.

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Keywords: Single-molecule Conformational Changes Shear Flow Fluorescence Microscopy Protein DNA Biophysics Microfluidic Device PDMS Surface Treatment Real-time Visualization Rheology Complex Fluids
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