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Cancer Research

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JoVE Journal Cancer Research
Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells
 

Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells

Article DOI: 10.3791/61399-v • 05:18 min • June 9th, 2020
June 9th, 2020 •
1, 1, 1, 1, 1
1Nuclear Facilities Operations Department, National Centre for Nuclear Research, Sołtana 7, 05-400 Otwock, Poland

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résumé
Automatic Translation

Radiation-induced DNA damage response activated by neutron mixed-beam used in boron neutron capture therapy (BNCT) has not been fully established. This protocol provides a step-by-step procedure to detect radiation-induced foci (RIF) of repair proteins by immunofluorescence staining in human colon cancer cell lines after irradiation with the neutron-mixed beam.

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Keywords: Immunofluorescence DNA Damage DNA Repair Colon Cancer Cells Neutron-mixed Beam BNCT Proton Beam Therapy Hadron Therapy Radiation-induced Foci Repair Proteins Gamma-H2AX Foci
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