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Biochemistry

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JoVE Journal Biochemistry
Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.)
 

Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.)

Article DOI: 10.3791/61440-v • 10:22 min • June 30th, 2020
June 30th, 2020 •
*1,4, *2,4, 3, 1,4
1Unidad de Hortofruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), 2Unidad de Producción y Sanidad Animal, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), 3Dpto Química Analítica, Escuela Politécnica Superior (Universidad de Zaragoza), 4Instituto Agroalimentario de Aragón - IA2 (CITA-Universidad de Zaragoza)
* These authors contributed equally

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We present a fast and reliable method to quantify vitamin C in Lactuca spp. using UPLC-UV, potentially transferable to other plants. The key steps are the sample preparation and vitamin C extraction under stable conditions, the reduction of dehydroascorbic acid to ascorbic acid and the optimization of the chromatographic procedure.

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Keywords: Vitamin C Lettuce Lactuca Sativa Lactuca Spp. UPLC-UV Quantification Extraction Ascorbic Acid Dehydroascorbic Acid Food Technology Nutrition Plant Breeding
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