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Developmental Biology

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JoVE Journal Developmental Biology
A Patient-Derived Xenograft Model for Venous Malformation
 

A Patient-Derived Xenograft Model for Venous Malformation

Article DOI: 10.3791/61501-v • 06:51 min • June 15th, 2020
June 15th, 2020 •
*1, *1, 1,2
1Division of Experimental Hematology and Cancer Biology, Cincinnati Children's Hospital Medical Center, 2Department of Pediatrics, University of Cincinnati College of Medicine
* These authors contributed equally

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résumé
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We present a detailed protocol to generate a murine xenograft model of venous malformation. This model is based on the subcutaneous injection of patient-derived endothelial cells containing hyper-activating TIE2 and/or PIK3CA gene mutations. Xenograft lesions closely recapitulate the histopathological features of VM patient tissue.

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Keywords: Patient-derived Xenograft Venous Malformation Preclinical Therapeutic Testing Endothelial Cells Vascular Anomalies Trypsinization Cell Suspension Centrifugation BMEM Anesthesia Injection Caliper Measurement Formalin Fixation
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