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Developmental Biology

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JoVE Journal Developmental Biology
Isolering av Murine Spermaogena celler med hjälp av en Violet-Excited Cell-Permeable DNA Bindande Dye
 
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Isolering av Murine Spermaogena celler med hjälp av en Violet-Excited Cell-Permeable DNA Bindande Dye

Article DOI: 10.3791/61666-v • 08:21 min • January 14th, 2021
January 14th, 2021 •
*1,2, *1,2, 3,4, 1,2, 3,4, 5,6, 1,2
1Division of Reproductive Sciences, Division of Developmental Biology, Perinatal Institute, Cincinnati Children's Hospital Medical Center, 2Department of Pediatrics, University of Cincinnati College of Medicine, 3Division of Germ Cell Biology, National Institute for Basic Biology, National Institutes of Natural Sciences, 4Department of Basic Biology, School of Life Science, Graduate University for Advanced Studies (Sokendai), 5Department of Animal Science and Biotechnology, School of Veterinary Medicine, Azabu University, 6Faculty of Science and Technology, Department of Applied Biological Science, Tokyo University of Science
* These authors contributed equally

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Här presenterar vi en enkel och effektiv metod för att isolera levande meiotiska och post-meiotiska könsceller från vuxna mustestes. Med hjälp av en lågcyttoxicitet, violett-upphetsad DNA bindande färgämne och fluorescens-aktiverad cell sortering, kan man isolera högberikad spermatogenic cell populationer för många nedströms applikationer.

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Utvecklingsbiologi Utgåva 167 spermatogenes meios spermatocyter spermatider fluorescens-aktiverad cellsortering (FACS) DyeCycle Violet (DCV)
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