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JoVE Journal Cancer Research
Isolering af proksimale væsker for at undersøge tumormikromiljøet af pancreas adenocarcinom
 
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Isolering af proksimale væsker for at undersøge tumormikromiljøet af pancreas adenocarcinom

Article DOI: 10.3791/61687-v • 05:44 min • November 5th, 2020
November 5th, 2020 •
*1, *2, 1,3, 1,3, 4,5,6, 1,3, 2,7, 2
1Section of Pancreatic Surgery, Humanitas Clinical and Research Center-IRCCS, 2Department of Immunology and Inflammation, Humanitas Clinical and Research Center-IRCCS, 3Department of Biomedical Sciences, Humanitas University, 4Giotto Biotech S.R.L., 5Section of Bioanalytical Chemistry, Division of Systems Medicine, Department of Metabolism, Digestion and Reproduction, Imperial College London, 6National Phenome Centre, Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Imperial College London, 7Department of Medical Biotechnology and Translational Medicine, University of Milan
* These authors contributed equally

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Bugspytkirtelsaft er en dyrebar kilde til biomarkører for kræft i bugspytkirtlen. Vi beskriver her en metode til intraoperativ indsamlingsprocedure. For at overvinde udfordringen med at vedtage denne procedure i murinemodeller foreslår vi en alternativ prøve, tumor interstitiel væske, og beskriver her to protokoller for dens isolering.

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Kræftforskning udgave 165 pancreas adenocarcinom bugspytkirtelsaft tumor interstitiel væske proksimal væske flydende biopsi biomarkører proteomics
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