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JoVE Journal Neuroscience
Semi-Quantitative Determination of Dopaminergic Neuron Density in the Substantia Nigra of Rodent Models using Automated Image Analysis
 

Semi-Quantitative Determination of Dopaminergic Neuron Density in the Substantia Nigra of Rodent Models using Automated Image Analysis

Article DOI: 10.3791/62062-v • 06:09 min • February 2nd, 2021
February 2nd, 2021 •
1,2, 1,2, 1,2, 1,2,3, 1,2,4,5,6
1Krembil Research Institute, Toronto Western Hospital, University Health Network, 2Department of Laboratory Medicine and Pathobiology, University of Toronto, 3Department of Surgery, Division of Neurosurgery, University of Toronto, 4Department of Medicine, Division of Neurology, University of Toronto, 5Department of Medicine, Division of Neurology, Edmond J. Safra Program in Parkinson's Disease and the Morton and Gloria Shulman Movement Disorders Clinic, Toronto Western Hospital, University Health Network, 6Tanz Centre for Research in Neurodegenerative Diseases, University of Toronto

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Here we present an automated method for semi-quantitative determination of dopaminergic neuron number in the rat substantia nigra pars compacta.

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Semi-Quantitative Determination Dopaminergic Neuron Density Substantia Nigra Rodent Models Automated Image Analysis Parkinson's Disease Research Neuron Number Estimation Time Efficient Process Accurate Process Interventions On Cell Survival Brain Regions Confocal Microscope IHC Images Tile Scan Imaging Option Dimensions Zoom Frame Size Averaging Channels Panel Alexa 488 Alexa 555 TH Staining Laser Strength
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