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Immunology and Infection

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JoVE Journal Immunology and Infection
Monitoring Neutrophil Elastase and Cathepsin G Activity in Human Sputum Samples
 

Monitoring Neutrophil Elastase and Cathepsin G Activity in Human Sputum Samples

Article DOI: 10.3791/62193-v • 09:23 min • May 21st, 2021
May 21st, 2021 •
*1,2, *1,2,3,4, 1,2,5,6,7, 1,3,8
1Translational Lung Research Center Heidelberg (TLRC), German Center for Lung Research (DZL), 2Dept. of Translational Pulmonology, University of Heidelberg, 3Molecular Medicine Partnership Unit (MMPU), European Molecular Biology Laboratory (EMBL), University of Heidelberg, 4Faculty of Biosciences, Collaboration for Joint Ph.D. Degree between EMBL and Heidelberg University, University of Heidelberg, 5Dept. of Pediatric Pulmonology, Immunology and Critical Care Medicine, Charité – Universitätsmedizin Berlin, 6Berlin Institute of Health (BIH), 7German Center for Lung Research (DZL), Associated Partner Site, Berlin, 8Dept. of Chemical Physiology and Biochemistry, Oregon Health and Science University
* These authors contributed equally

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résumé
Automatic Translation

The protocols herein described provide a guide to visualize and quantify the activity of neutrophil proteases in human sputum. The applications of such analysis span from the evaluation of anti-inflammatory treatments, to biomarker validation, drug screening and large cohort clinical studies.

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Monitoring Neutrophil Elastase Cathepsin G FRET Probe Protocols Quantification Sputum Samples Proteases Pathophysiology Plate Reader Measurements Confocal Microscopy Flow Cytometry Airway Diseases Cystic Fibrosis Bronchiectasis COPD BioSamples Blood Samples Bronchial Alveola Lavash Mice Samples Sputum Induction Procedure Beta 2 Receptor Antagonists Salbutamol Hypertonic 6% Saline Solution Expectorated Sputum Mucus Clumps Sputolysin PBS Solution
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