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Cancer Research

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JoVE Journal Cancer Research
Fluorescence Microscopy for ATP Internalization Mediated by Macropinocytosis in Human Tumor Cells and Tumor-xenografted Mice
 

Fluorescence Microscopy for ATP Internalization Mediated by Macropinocytosis in Human Tumor Cells and Tumor-xenografted Mice

Article DOI: 10.3791/62768-v • 08:31 min • June 30th, 2021
June 30th, 2021 •
*1,2,3, *4, 1,5, 4, 1,2,4, 1,2,4, 6, 1,2,4,7
1Department of Biological Sciences, Ohio University, 2Molecular and Cellular Biology Program, Ohio University, 3Neuroscience Program, Ohio University, 4Edison Biotechnology Institute, Ohio University, 5Translational Biomedical Sciences Program, Ohio University, 6Honors Tutorial College, Ohio University, 7Department of Biomedical Sciences, Heritage College of Osteopathic Medicine, Ohio University
* These authors contributed equally

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résumé
Automatic Translation

We developed a reproducible method to visualize the internalization of nonhydrolyzable fluorescent adenosine triphosphate (ATP), an ATP surrogate, with high cellular resolution. We validated our method using independent in vitro and in vivo assays-human tumor cell lines and immunodeficient mice xenografted with human tumor tissue.

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Fluorescence Microscopy ATP Internalization Macropinocytosis Human Tumor Cells Tumor-xenografted Mice High Resolution Imaging ATP Localization Macropynozomes Experimental Applications Mechanisms Of Cellular Internalization Metabolic Diseases Cancer Cells Cell Culture Incubator Centrifugation Cell Density Immunodeficient Mouse
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