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JoVE Journal Bioengineering
Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System (MMC)
 

Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System (MMC)

Article DOI: 10.3791/62800-v • 08:18 min • February 18th, 2022
February 18th, 2022 •
*1,2, *1,2,3, 4, 1,2,6, 1,2,5, 3, 1,2,5
1Department of Chemical Engineering, Institute of Biochemical Engineering, Tsinghua University, 2Key Laboratory of Industrial Biocatalysis, Ministry of Education, Tsinghua University, 3Luoyang TMAXTREE Biotechnology Co., Ltd., 4Biochemical Engineering Research Group, School of Chemical Engineering and Technology, Xi’an Jiaotong University, 5Center for Synthetic & Systems Biology, Tsinghua University, 6School of Life Science and Technology, Tokyo Institute of Technology
* These authors contributed equally

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résumé
Automatic Translation

This protocol describes how to use the Microbial Microdroplet Culture system (MMC) to conduct automated microbial cultivation and adaptive evolution. MMC can cultivate and sub-cultivate microorganisms automatically and continuously and monitor online their growth with relatively high throughput and good parallelization, reducing labor and reagent consumption.

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Keywords: Automated Microbial Cultivation Adaptive Evolution Microbial Microdroplet Culture System (MMC) Low-cost Operation-friendly Reliable Results Simple Operations Microbiology Research Growth Curve Measurement Adaptive Laboratory Evolution Single Factor Multi-level Analysis Microfluidic Chip Escherichia Coli MG1655 Cultivation Temperature Photoelectric Signal MMC Oil Initial Bacteria Solution
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