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JoVE Journal Neuroscience
Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis
 

Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis

Article DOI: 10.3791/62813-v • 08:59 min • July 16th, 2021
July 16th, 2021 •
*1, 1, 1, *1
1Jefferson Weinberg ALS Center, Thomas Jefferson University
* These authors contributed equally

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Two related methods are described to visualize subcellular events required for synaptic transmission. These protocols enable the real-time monitoring of the dynamics of presynaptic calcium influx and synaptic vesicle membrane fusion using live-cell imaging of in vitro cultured neurons.

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Keywords: Real-time Fluorescent Synaptic Amyotrophic Lateral Sclerosis Synaptic Function Electrophysiology Primary Rodent Neuronal Cultures Induced Pluripotent Stem Cells GCaMP Styryl Dye ACSF Potassium Chloride Confocal Imaging
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