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Isolation and Culture of Primary Oral Keratinocytes from the Adult Mouse Palate
 

Isolation and Culture of Primary Oral Keratinocytes from the Adult Mouse Palate

Article DOI: 10.3791/62820-v • 06:28 min • September 24th, 2021
September 24th, 2021 •
1,2,3, 4, 4, 4, 1,5, 4, 1,3
1Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, 2Ph.D. Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba, 3International Research Center for Medical Sciences (IRCMS), Kumamoto University, 4Division of Biomimetics, Faculty of Dentistry and Graduate School of Medical and Dental Sciences, Niigata University, 5Faculty of Medicine, University of Tsukuba

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résumé
Automatic Translation

The present protocol describes the isolation and culture of oral keratinocytes derived from the adult mouse palate. An evaluation method using immunostaining is also reported.

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Keywords: Primary Oral Keratinocytes Mouse Palate Stem Cell-like State Long-term Culture Epithelial Layer Trypsin Digestion Lamina Propria Tissue Harvesting Cell Isolation Culture Technique
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