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Immunology and Infection

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JoVE Journal Immunology and Infection
Preparation of Bead-supported Lipid Bilayers to Study the Particulate Output of T Cell Immune Synapses
 

Preparation of Bead-supported Lipid Bilayers to Study the Particulate Output of T Cell Immune Synapses

Article DOI: 10.3791/63130-v • 11:06 min • April 1st, 2022
April 1st, 2022 •
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1Kennedy Institute of Rheumatology, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, The University of Oxford

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Here we present the protocol for the stepwise reconstitution of synthetic antigen-presenting cells using Bead-Supported Lipid Bilayers and their use to interrogate the synaptic output from activated T cells.

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Keywords: Bead-supported Lipid Bilayers Immune Synapse Flow Cytometry Microscopy Proteomics RNA Sequencing Chimeric Antigen Receptors Signaling Pathways Genetic Ablation Protein Calibration Multicolor Flow Cytometry Silica Beads Liposome Nickel-containing Phospholipids Argon Nitrogen Blocking BSA Nickel Sulfate HBS-HSA Buffer Protein Dilutions
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