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Bioengineering

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Autofluorescence Imaging to Evaluate Cellular Metabolism
 

Autofluorescence Imaging to Evaluate Cellular Metabolism

Article DOI: 10.3791/63282-v 07:36 min November 15th, 2021
November 15th, 2021

Chapitres

résumé

This protocol describes fluorescence imaging and analysis of the endogenous metabolic coenzymes, reduced nicotinamide adenine (phosphate) dinucleotide (NAD(P)H), and oxidized flavin adenine dinucleotide (FAD). Autofluorescence imaging of NAD(P)H and FAD provides a label-free, nondestructive method to assess cellular metabolism.

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Keywords: Autofluorescence NADH FAD Cellular Metabolism Multi-photon Fluorescence Lifetime Microscopy Live Cell Imaging Non-destructive
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