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Genome-wide Profiling of Transcription Factor-DNA Binding Interactions in Candida albicans
 

Genome-wide Profiling of Transcription Factor-DNA Binding Interactions in Candida albicans: A Comprehensive CUT&RUN Method and Data Analysis Workflow

Article DOI: 10.3791/63655-v • 07:48 min • April 1st, 2022
April 1st, 2022 •
*1,2, *1,2, *1,2, 1,2,4, 3, 1,4, 1,4
1Department of Molecular and Cell Biology, University of California, Merced, 2Quantitative and Systems Biology Graduate Program, University of California, Merced, 3Department of Applied Mathematics, University of California, Merced, 4Health Sciences Research Institute, University of California, Merced
* These authors contributed equally

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This protocol describes an experimental method and data analysis workflow for cleavage under targets and release using nuclease (CUT&RUN) in the human fungal pathogen Candida albicans.

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Keywords: Genome-wide Profiling Transcription Factor-DNA Binding Candida Albicans CUT&RUN ChIP-chip ChIP-seq Epitope Tagging Nuclei Isolation Fluorescence Microscopy Antibody Incubation Protein AG-MNase Data Analysis Workflow
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