Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Isolation and Identification of Vascular…
JoVE Journal
Biology

Ce contenu est disponible gratuitement.

JoVE Journal Biology
Isolation and Identification of Vascular Endothelial Cells from Distinct Adipose Depots for Downstream Applications
 

Isolation and Identification of Vascular Endothelial Cells from Distinct Adipose Depots for Downstream Applications

Article DOI: 10.3791/63999-v • 09:00 min • June 10th, 2022
June 10th, 2022 •
1, 2, 3, 1
1Department of Kinesiology and Applied Physiology, College of Health Sciences, University of Delaware, 2Division of Pulmonary, Critical Care, Sleep and Allergy, Department of Medicine, University of Illinois at Chicago, 3University of Delaware Flow Cytometry Core, Delaware Biotechnology Institute, University of Delaware

Chapitres

résumé
Automatic Translation

This protocol details a method for the dissection of mouse adipose depots and the isolation and digestion of respective arteries to liberate and then identify the endothelial cell population. Freshly isolated cells used in downstream applications will advance the understanding of vascular cell biology and the mechanisms of vascular dysfunction.

Tags

Keywords: Vascular Endothelial Cells Adipose Tissue Vascular Beds Vascular Cell Biology Vascular Dysfunction Tissue Dissection Adipose Depot Isolation HEPES Buffer Peritoneal Cavity
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter