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Bioengineering

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JoVE Journal Bioengineering
Experimental Quantification of Interactions Between Drug Delivery Systems and Cells In Vitro
 

Experimental Quantification of Interactions Between Drug Delivery Systems and Cells In Vitro: A Guide for Preclinical Nanomedicine Evaluation

Article DOI: 10.3791/64259-v • 08:47 min • September 28th, 2022
September 28th, 2022 •
1, 2, 2,3,4, 5, 6
1Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, 2Department of Infectious Diseases, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, 3Victorian Infectious Diseases Service, Royal Melbourne Hospital at the Peter Doherty Institute for Infection and Immunity, 4Department of Infectious Diseases, Alfred Hospital and Monash University, 5Department of Chemical Engineering, The University of Melbourne, 6Department of Biomedical Engineering, The University of Melbourne

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résumé
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A workflow is demonstrated for the absolute quantification of drug carrier-cell interactions using flow cytometry to allow better rational evaluation of novel drug delivery systems. This workflow is applicable to drug carriers of any type.

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Keywords: Quantitative Analysis Drug Delivery Systems Cells In Vitro Nanoparticle Tracking Analysis Carrier Systems Preclinical Nanomedicine Evaluation Carrier Performance Linear Range Quantitative Range Flow Cell Carrier Debris Carrier Suspension Carrier Dilution Carrier Concentration
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