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Immunology and Infection

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JoVE Journal Immunology and Infection
Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ
 

Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ

Article DOI: 10.3791/64263-v • 10:39 min • August 9th, 2022
August 9th, 2022 •
1, 1, 1
1Risk Analysis and Genomic Epidemiology Unit, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna (IZSLER)

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résumé
Automatic Translation

Salmonella invades and replicates inside intestinal epithelial cells both in Salmonella-specific vacuoles and free in the cytosol (hyper-replication). A high-throughput fluorescence microscopy-based protocol is described here to quantify the intracellular phenotypes of Salmonella by two complementary image analyses through ImageJ, reaching single-cell resolution and scoring.

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Keywords: Fluorescence Microscopy Salmonella Intracellular Phenotypes ImageJ Automated Analysis High-throughput Single-cell Analysis Cell Segmentation Contrast Enhancement Maxima Detection Cell Masking Thresholding
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