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Biochemistry

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JoVE Journal Biochemistry
Levende cellebilleddannelse af intakte ex vivo glober ved hjælp af en ny 3D-trykt holder
 
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Levende cellebilleddannelse af intakte ex vivo glober ved hjælp af en ny 3D-trykt holder

Article DOI: 10.3791/64510-v • 05:41 min • October 6th, 2022
October 6th, 2022 •
*1, *2, 2, 3, 2,3
1Department of Pharmacology, School of Medicine, Boston University, 2Department of Biochemistry, School of Medicine, Boston University, 3Department of Ophthalmology, School of Medicine, Boston University
* These authors contributed equally

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Det nuværende arbejde beskriver en ny eksperimentel protokol, der bruger en 3D-trykt holder til at muliggøre højopløsnings levende cellebilleddannelse af enukleerede kloder. Gennem denne protokol kan den cellulære calciumsignalaktivitet i såret hornhindeepitel fra ex vivo-kloder observeres i realtid.

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