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Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae
 

Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae

Article DOI: 10.3791/64580-v • 07:48 min • October 11th, 2022
October 11th, 2022 •
*1, *1, 1,2, 1
1Department of Biology, Indiana University, 2Stowers Institute for Biomedical Research
* These authors contributed equally

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résumé
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Time-lapse microscopy is a valuable tool for studying meiosis in budding yeast. This protocol describes a method that combines cell-cycle synchronization, time-lapse microscopy, and conditional depletion of a target protein to demonstrate how to study the function of a specific protein during meiotic chromosome segregation.

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Keywords: Time-lapse Microscopy Stage-specific Nuclear Depletion Meiosis Saccharomyces Cerevisiae Prophase I Conditional Protein Depletion Budding Yeast Mitosis Cell Cycle Events Nuclear Envelope Breakdown Agar Pad Monolayer Of Cells Sporulation Culture Centrifugation
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