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Developmental Biology

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JoVE Journal Developmental Biology
Differentiation of Human Pluripotent Stem Cells into Insulin-Producing Islet Clusters
 

Differentiation of Human Pluripotent Stem Cells into Insulin-Producing Islet Clusters

Article DOI: 10.3791/64840-v • 08:41 min • June 23rd, 2023
June 23rd, 2023 •
*1, *1, 1, 1, 1,2, 3, 1,2,4
1Department of Cellular & Physiological Sciences, Life Science Institute, University of British Columbia, 2School of Biomedical Engineering, University of British Columbia, 3STEMCELL Technologies Inc, 4Department of Surgery, University of British Columbia
* These authors contributed equally

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résumé
Automatic Translation

The differentiation of stem cells into islet cells provides an alternative solution to conventional diabetes treatment and disease modeling. We describe a detailed stem cell culture protocol that combines a commercial differentiation kit with a previously validated method to aid in producing insulin-secreting, stem cell-derived islets in a dish.

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Keywords: Human Pluripotent Stem Cells Insulin-producing Islet Clusters Static Suspension Culture Pancreatic Progenitors Islet-like Clusters HESC-qualified Matrigel Cell Dissociation Stem Cell Culture Stage 1A Medium Stage 1B Medium Stage 2A Medium Stage 2B Medium Stage 3 Medium Stage 4 Medium Anti-adherence Rinsing Solution Microwell Plate
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