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Developmental Biology

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JoVE Journal Developmental Biology
Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing
 

Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing

Article DOI: 10.3791/55702-v • 09:02 min • June 9th, 2017
June 9th, 2017 •
1, 1, 1, 1
1Directorate F - Health, Consumers and Reference Materials, Joint Research Centre

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Human induced pluripotent stem cells (hiPSCs) are considered a powerful tool for drug and chemical screening and for the development of new in vitro models for toxicity testing, including neurotoxicity. Here, a detailed protocol for the differentiation of hiPSCs into neurons and glia is described.

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Keywords: Human Induced Pluripotent Stem Cells Neural Stem Cells Neuronal Differentiation Glial Differentiation Mixed Cultures Neurotoxicity Testing In Vitro Model Drug And Chemical Toxicity Screening Signaling Pathways HiPSC Medium EB Medium Colony Fragments Matrix-coated Dishes
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