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Developmental Biology

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JoVE Journal Developmental Biology
Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays
 

Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays

Article DOI: 10.3791/54900-v • 09:20 min • January 8th, 2017
January 8th, 2017 •
*1,2, *3, 2,3, 3, 1,2, 1,2, 1,2, 2,3,4, 1,2,3
1Department of Cognitive Neurosciences, Radboudumc, 2Donders Institute for Brain, Cognition and Behaviour, Radboud University, 3Department of Human Genetics, Radboudumc, 4Department of Molecular Developmental Biology, Radboud University
* These authors contributed equally

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We modify and implement a previously published protocol describing the rapid, reproducible, and efficient differentiation of human induced Pluripotent Stem Cells (hiPSCs) into excitatory cortical neurons12. Specifically, our modification allows for control of neuronal cell density and use on micro-electrode arrays to measure electrophysiological properties at the network level.

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Keywords: Neuronal Differentiation Induced Pluripotent Stem Cells Micro-electrode Arrays Neurological Disorders Neurobiology Rapid Differentiation DMEM/F12 CDS E8 Medium Doxycycline ROCK Inhibitor HiPSCs Cell Suspension Laminin MEAs Cell Plating
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