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Methylated DNA Immunoprecipitation
 

Methylated DNA Immunoprecipitation

Article DOI: 10.3791/935-v • 21:25 min • January 2nd, 2009
January 2nd, 2009 •
*1,2, *1,3, 1,3, 5, 5, 1,2,3, 1,3
1Department of Cancer Genetics and Developmental Biology, BC Cancer Research Centre, 2Interdisciplinary Oncology Program, University of British Columbia - UBC, 3Department of Pathology and Laboratory Medicine, University of British Columbia - UBC, 4Photography/Video Production, Multi-Media Services, BC Cancer Agency, 5Department of Medical Genetics, Life Sciences Institute,, University of British Columbia - UBC
* These authors contributed equally

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résumé
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This video demonstrates the protocol for methylated DNA immunoprecipitation (MeDIP). MeDIP is a two day procedure that selectively extracts methylated DNA fragments from a genomic DNA sample using antibodies with specificity for 5 -methylcytosine (anti-5 mC).

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Methylated DNA Immunoprecipitation DNA Methylation Patterns Epigenetics Gene Expression Normal Development Disease Methylation Profiles MeDIP Methylated DNA Extraction Antibody Reaction Immunoprecipitated DNA Input DNA Heat Denaturation Anti-5'mC Antibody Magnetic Beads Secondary Antibody Antibody Complex Separation Of Methylated DNA Non-methylated DNA Removal
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