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Chapters
- 00:05Title
- 01:12Treatment of HeLa Cells with Biotinylated Psoralen RNA Extraction, and Fragmentation
- 02:25RNA Size Selection and Elution
- 04:41Enrichment of RNA Crosslinking Regions
- 05:57Proximity Ligation
- 07:34Reverse Crosslinking of Biotinylated Psoralen, and Amplification of cDNA
- 09:13The SPLASH Workflow and Representative Results
- 10:50Conclusion
Her beskriver vi metoden for S- ækvurdering af P soralen tværbundet, L igateret og S valgte H ybrids (SPLASH), som muliggør genomgående kortlægning af intramolekylære og intermolekylære RNA-RNA-interaktioner in vivo . SPLASH kan anvendes til at studere RNA-interaktomer af organismer, herunder gær, bakterier og mennesker.
