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Single-molecule Imaging of Gene Regulation In vivo Using Cotranslational Activation by Cleavage (CoTrAC)
 

Single-molecule Imaging of Gene Regulation In vivo Using Cotranslational Activation by Cleavage (CoTrAC)

Article DOI: 10.3791/50042-v • 11:31 min • March 15th, 2013
March 15th, 2013 •
1, 1,2,3, 1
1Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, 2Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 3Department of Physics, Jilin University

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We describe a fluorescence microscopy method, Co-Translational Activation by Cleavage (CoTrAC), to image the production of protein molecules in live cells with single-molecule precision without perturbing the protein's functionality. This method has been used to follow the stochastic expression dynamics of a transcription factor, the λ repressor CI 1.

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Keywords: Single-molecule Imaging Gene Regulation In Vivo Cotranslational Activation By Cleavage (CoTrAC) Fluorescence Microscopy Protein Production Live Cells Fluorescent Reporter Membrane Targeting Protease Recognition Protein Of Interest Fluorescence Imaging Photobleaching Cell Lineages Gene Expression Lambda Repressor CI Systems Biology
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