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Bioengineering

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JoVE Journal Bioengineering
Helical Organization of Blood Coagulation Factor VIII on Lipid Nanotubes
 

Helical Organization of Blood Coagulation Factor VIII on Lipid Nanotubes

Article DOI: 10.3791/51254-v • 12:24 min • June 3rd, 2014
June 3rd, 2014 •
*1, *1, 2,3, 1, 1,3
1Department of Neuroscience and Cell Biology, University of Texas Medical Branch, 2Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, 3Sealy Center for Structural Biology and Molecular Biophysics, University of Texas Medical Branch
* These authors contributed equally

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We present a combination of Cryo-electron microscopy, lipid nanotechnology, and structure analysis applied to resolve the membrane-bound structure of two highly homologous FVIII forms: human and porcine. The methodology developed in our laboratory to helically organize the two functional recombinant FVIII forms on negatively charged lipid nanotubes (LNT) is described.

Tags

Cryo-electron Microscopy Lipid Nanotubes Coagulation Factor VIII FVIII Hemophilia Type A Serine Protease Blood Clotting Membrane-bound Structure Recombinant FVIII Human Factor IXa Porcine FVIII Structure Analysis
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