Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Measuring the Osmotic Water Permeability…
JoVE Journal
Biology

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Biology
Measuring the Osmotic Water Permeability Coefficient (Pf) of Spherical Cells
 

Measuring the Osmotic Water Permeability Coefficient (Pf) of Spherical Cells: Isolated Plant Protoplasts as an Example

Article DOI: 10.3791/51652-v • 14:20 min • October 8th, 2014
October 8th, 2014 •
1, 1, 2, 3, 1, 1
1The RH Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, 2Earth and Life Institute, Université catholique de Louvain, 3Institut des Sciences de la Vie, Université catholique de Louvain

Capitoli

Riepilogo
Automatic Translation

Measuring the osmotic water permeability coefficient (Pf) of cells can help understand the regulatory mechanisms of aquaporins (AQPs). Pf determination in spherical plant cell protoplasts presented here involves protoplasts isolation and numerical analysis of their initial rate of volume change as a result of an osmotic challenge during constant bath perfusion.

Tags

Osmotic Water Permeability Plant Protoplasts AQP Regulation Cell Volume Changes Hypotonic Solution Osmotic Challenge Assay Pf Measurement
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter