Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / A Proteoliposome-Based Efflux Assay to Determine…
JoVE Journal
Biology

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Biology
A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl- Channels and Transporters
 

A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl- Channels and Transporters

Article DOI: 10.3791/52369-v • 07:47 min • April 20th, 2015
April 20th, 2015 •
1, 1,2,3
1Department of Anesthesiology, Weill Cornell Medical College, 2Department of Physiology and Biophysics, Weill Cornell Medical College, 3Department of Biochemistry, Weill Cornell Medical College

Capitoli

Riepilogo
Automatic Translation

Proteoliposomes are used to study purified channels and transporters reconstituted in a well-defined biochemical environment. An experimental procedure to measure efflux mediated by these proteins is illustrated. The steps to prepare proteoliposomes, perform the recordings, and analyze data to quantitatively determine the functional properties of the reconstituted protein are described.

Tags

Keywords: Proteoliposome Efflux Assay Cl- Channels Transporters Membrane Proteins Ion Sensitive Electrode CLC-ec1 H+/Cl- Exchanger Ion Transport Quantitative Analysis Structure-function Studies Small Molecule Effects Membrane Composition Effects
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter