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Developmental Biology
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Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures
 

Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures

Article DOI: 10.3791/52485-v 07:35 min March 7th, 2015
March 7th, 2015

Capitoli

Riepilogo

Mature adipocytes may represent an abundant source of stem cells through dedifferentiation, which leads to a homogenous population of fibroblast-like cells. Collagenase digestion is used to isolate mature adipocytes from human fat. The goal of our protocol is to obtain multipotent, dedifferentiated fat cells from human mature adipocytes.

Tags

Adipose Stem Cells Mature Adipocytes Ceiling Culture Dedifferentiation FABP4 Adipose Tissue Cell Isolation Collagenase Digestion Cell Culture
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