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Developmental Biology

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JoVE Journal Developmental Biology
Generation of Airway Epithelial Cell Air-Liquid Interface Cultures from Human Pluripotent Stem Cells
 

Generation of Airway Epithelial Cell Air-Liquid Interface Cultures from Human Pluripotent Stem Cells

Article DOI: 10.3791/63882-v • 10:46 min • June 14th, 2022
June 14th, 2022 •
1,2, 2, 3, 2, 2, 3, 1,2, 1,2
1Pulmonary Center, Boston University School of Medicine, 2Center for Regenerative Medicine, Boston University and Boston Medical Center, 3Center for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center

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Recent advances in human induced pluripotent stem cell differentiation protocols allow for the stepwise derivation of organ-specific cell types. Here, we provide detailed steps for the maintenance and expansion of iPSC-derived airway basal cells and their differentiation into a mucociliary epithelium in air-liquid interface cultures.

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Keywords: Airway Epithelial Cells Air-liquid Interface Human Pluripotent Stem Cells 3D Matrix Cell Culture Cell Differentiation Basal Cell Medium Dispase II Dissociation
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