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JoVE Journal
Medicine

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Intravital Microscopy of Tumor-associated Vasculature Using Advanced Dorsal Skinfold Window Chambers on Transgenic Fluorescent Mice
 

Intravital Microscopy of Tumor-associated Vasculature Using Advanced Dorsal Skinfold Window Chambers on Transgenic Fluorescent Mice

Article DOI: 10.3791/55115-v 08:52 min January 19th, 2018
January 19th, 2018

Capitoli

Riepilogo

This protocol describes the intravital imaging of transgenic mice expressing cell-specific fluorescent markers. Intravital imaging provides a non-invasive method for high-resolution observations in living animals using implantable windows through which the microscopic visualization of processes is possible. This method is especially useful to study longitudinal processes.

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Keywords: Intravital Microscopy Tumor Vasculature Dorsal Skinfold Window Chamber Transgenic Fluorescent Mice Tumor Implantation Syngeneic Tumor Xenograft Surgical Procedure Aseptic Conditions Hair Removal Skin Preparation Frame Installation Glass Filler Cover Glass
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