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Immunology and Infection

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JoVE Journal Immunology and Infection
En Fluorescens-baserede lymfocyt Assay Egnet til high-throughput screening af små molekyler
 
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En Fluorescens-baserede lymfocyt Assay Egnet til high-throughput screening af små molekyler

Article DOI: 10.3791/55199-v • 08:43 min • March 10th, 2017
March 10th, 2017 •
1, 2, 2, 2, 1,2,3
1Department of Microbiology, Infectiology and Immunology, Université de Montréal, 2Department of Pharmacology and Physiology, Université de Montréal, 3Molecular Biology Program, Université de Montréal

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Vi præsenterer i den aktuelle undersøgelse en hidtil ukendt fluorescens-baseret assay under anvendelse af lymfocytter afledt fra en transgen mus. Dette assay er egnet til high-throughput screening (HTS) af små molekyler forsynet med kapacitet på enten inhibere eller fremme lymfocytaktivering.

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Immunologi High-throughput screening fænotypisk screening target-baseret screening drug discovery lymfocytter T-celler B-celler fluorescens grønt fluorescerende protein Nur77 immunosuppression aktivering
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