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Journal / Biochemistry / Recombinant Protein Expression, Crystallization,…
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Biochemistry

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JoVE Journal Biochemistry
Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG
 

Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG

Article DOI: 10.3791/55576-v • 12:23 min • May 16th, 2017
May 16th, 2017 •
1, 1, 1
1Division of Biological Science and Technology, Yonsei University

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Bacillus anthracis is the obligate pathogen of fatal inhalational anthrax, so studies of its genes and proteins are strictly regulated. An alternative approach is to study orthologous genes. We describe biophysicochemical studies of a B. anthracis ortholog in B. cereus, bc1531, requiring minimal experimental equipment and lacking serious safety concerns.

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Keywords: Recombinant Protein Bacillus Nucleotide Pyrophosphorylase BcMazG Protein Expression Protein Crystallization Biophysical Studies E. Coli Chemically Competent Cells Transformation Plasmid DNA DNA Sequencing Protein Purification IPTG Induction Cell Lysis Protein Characterization
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