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Plant Promoter Analysis
 

Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean

Article DOI: 10.3791/56140-v 10:58 min December 23rd, 2017
December 23rd, 2017

Capitoli

Riepilogo

Promoter expression analyses are crucial to improving the understanding of gene regulation and the spatiotemporal expression of target genes. Herein we present a protocol to identify, isolate, and clone a plant promoter. Further, we describe the characterization of the nodule-specific promoter in the common bean hairy roots.

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Keywords: Plant Promoter Root Nodule Hairy Root Phaseolus Reporter Gene Agrobacterium Rhizogenes NIN Gene PCR Gateway Cloning Binary Vector
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