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JoVE Journal Cancer Research
Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation
 

Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation

Article DOI: 10.3791/56418-v • 09:09 min • November 10th, 2017
November 10th, 2017 •
*1, *1, 2, 1
1Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, 2Department of Experimental Therapeutics, University of Texas MD Anderson Cancer Center
* These authors contributed equally

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This protocol describes the use of centrifugal elutriation to separate primary acute lymphoblastic leukemia cells into different cell cycle phases.

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Keywords: Primary Acute Lymphoblastic Leukemia Cell Cycle Phases Centrifugal Elutriation Cell Separation Cancer Therapeutics Anti-cancer Compounds Cell Isolation Physical Properties Cell Cycle Strobe Assembly Rotor Elutriation Chamber Rotating Seal Assembly Flow System Buffer Variable Speed Pump Sterilization 5% Bleach
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