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Genetics

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JoVE Journal Genetics
Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II
 

Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II

Article DOI: 10.3791/56736-v • 10:39 min • February 26th, 2018
February 26th, 2018 •
*1, *1, 1
1Department of Biochemistry and Molecular Biology, Johns Hopkins University Bloomberg School of Public Health
* These authors contributed equally

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Oogenesis in mammals is known to be error-prone, particularly due to chromosome missegregation. This manuscript describes chromatin spread preparation methods for mouse prophase, metaphase I and II-staged oocytes. These fundamental techniques allow for the study of chromatin-bound proteins and chromosome morphology throughout mammalian oogenesis.

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Chromatin Spread Preparations Mouse Oocyte Progression Prophase To Metaphase II Chromatin-bound Proteins Chromosome Morphology Mammalian Oogenesis Female Reproductive Biology Homologous Chromosome Pairing Synapsis DNA Repair Meiotic Chromosome Segregation Immunolabeling Oocyte Ploidy Aneuploidy Infertility Maternal Causes Euthanizing Abdominopelvic Cavity Uterine Horn Embryos Decapitation Petri Dish PBS Warm PBS
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