Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Zygotic Fluorescence Recovery After…
JoVE Journal
Developmental Biology

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Developmental Biology
Zygotic Fluorescence Recovery After Photo-bleaching Analysis for Chromatin Looseness That Allows Full-term Development
 

Zygotic Fluorescence Recovery After Photo-bleaching Analysis for Chromatin Looseness That Allows Full-term Development

Article DOI: 10.3791/57068-v • 10:30 min • June 12th, 2018
June 12th, 2018 •
1,3, 2, 2, 1,3
1Faculty of Life and Environmental Sciences, Department of Biotechnology, University of Yamanashi, 2Department of Integrated Bioscience, Graduate School of Frontier Sciences, University of Tokyo, 3Advanced Biotechnology Center, University of Yamanashi

Capitoli

Riepilogo
Automatic Translation

Chromatin looseness appears to be involved in the developmental potential of blastomeres. However, it is not known whether chromatin looseness can be used as a reliable index for the developmental potential for embryos. Here, an experimental system in which chromatin looseness-evaluated zygotes can develop to full term has been described.

Tags

Keywords: Zygotic FRAP ZFRAP Chromatin Looseness Embryonic Development EGFP-H2B MRNA Injection Microinjection Confocal Microscopy Photo-bleaching
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter