Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Bioengineering / A Droplet-Based Microfluidic Approach and…
JoVE Journal
Bioengineering

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Bioengineering
A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
 

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Article DOI: 10.3791/57703-v • 11:40 min • November 14th, 2018
November 14th, 2018 •
*1, *2, 2, 3, 3, 1, 2,3, 1
1College of Natural Sciences, Chungbuk National University, 2Department of Biomedical Engineering, Inje University, 3Institute of Digital Anti-Aging Healthcare, Inje University
* These authors contributed equally

Capitoli

Riepilogo
Automatic Translation

This work provides a method for the fabrication of droplet-based microfluidic platforms and the application of polyacrylamide microspheres for microsphere-PCR amplification. The microsphere-PCR method makes it possible to obtain single-stranded DNA amplicons without separating double-stranded DNA.

Tags

Keywords: Microfluidic Microsphere PCR Single-stranded DNA MEMS PDMS Flow-focusing Hydrophilic Corona Treater Acrydite Acrylamide
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter